anti coup tfii (R&D Systems)

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R&D Systems anti coup tfii
Anti Coup Tfii, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+coup+tfii/Human+COUP-TF+II%2FNR2F2+Antibody/pmc12758517-364-27-29
Average 94 stars, based on 80 article reviews

anti coup tfii - by Bioz Stars, 2026-09

94/100 stars

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Chromatin Immunoprecipitation:

Article Title: An expanded subventricular zone supports postnatal cortical interneuron migration in gyrencephalic brains
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used anti-ALDH1L1 (mouse, N103/39 clone, Antibodies Inc, 75-140, 1:500 dilution) anti-a-SMA (mouse, 1A4 clone, Sigma, a2547, 1:500 dilution) anti-COUP-TFII (mouse, H7147 clone, R&D Systems, PP-H7147-00, 1:100 dilution) anti-BLBP (rabbit, Abcam, ab32423, 1:500 dilution). anti-doublecortin (rabbit, Cell Signaling, 4604, 1:200 dilution). anti-doublecortin (guinea Pig, Millipore, ab2253, 1:200 dilution). anti-DLX2 (mouse, 3B6 clone, Santa Cruz, sc-393879, 1:200 dilution). anti-EMX1 (mouse, 3H2 clone, Santa Cruz, sc-398115, 1:200 dilution). anti-FOXP2 (rabbit, Millipore, ABE74, 1:500 dilution). anti-GFAP (chicken, Abcam, ab4674, 1;750 dilution). anti-GFP (chicken IgY, Aves labs, GFP-1020, 1:1000 dilution). anti-GSH2 (rabbit, Abcam, ab26255, 1:200 dilution). anti-IBA1 (goat, Abcam, ab5076, 1:250 dilution). anti-LHX6 (mouse, 6F3 clone, Santa Cruz, sc-271433, 1:200 dilution). anti-MEIS2 (mouse, 2F4 clone, Sigma,WH0004212M1, 1:500 dilution). anti-Mki67 (rabbit, Abcam, ab15580, 1:500 dilution). anti-NeuN (chicken, Millipore, ABN91, 1:500 dilution). anti-NKX2.1 (rabbit, Santa Cruz, sc-13040, 1:100 dilution). anti-Olig2 (rabbit, Millipore, AB9610, 1:200 dilution). anti-MBP (rat, 2D1 clone, Millipore, MAB386, 1:200 dilution). anti-PAX6 (mouse, 1B5 clone, Abcam, ab78545, 1:200 dilution). anti-PROX1 (goat, R&D, AF2727, 1:200 dilution). anti-PSA-NCAM (mouse, 5A5 clone, Millipore, MAB5324, 1:500 dilution). anti-SCGN (mouse, EPR23393-30 clone, Abcam, ab244219, 1:500 dilution) anti-SP8 (goat, Santa Cruz, sc-10466, 1:100 dilution). anti-TSHZ1 (rabbit, Abcam, ab140196, 1:200 dilution). anti-CXCR4 (rabbit, Abcam, ab124824, 1:100 dilution). anti-VLDLR (rabbit, Abcam, ab203271, 1:200 dilution). anti-PV (rabbit, Swant, PV27, 1:500 dilution). anti-PV (mouse, 1B2 clone, Swant, PV235, 1:200 dilution). anti-SST (rat, Abcam, ab30788, 1:200 dilution). anti-VIP (rabbit, Abcam, ab22736, 1:200 dilution). anti-CALB2 (rabbit, Swant, 7697, 1:200 dilution). anti-CALB2 (guinea Pig, Swant, CRgp7, 1:200 dilution). .. Biotin-SP-AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 715065151, 1:250 dilution).

Article Title: An expanded subventricular zone supports postnatal cortical interneuron migration in gyrencephalic brains.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used anti-ALDH1L1 (mouse, N103/39 clone, Antibodies Inc, 75-140, 1:500 dilution) anti-a-SMA (mouse, 1A4 clone, Sigma, a2547, 1:500 dilution) anti-COUP-TFII (mouse, H7147 clone, R&D Systems, PP-H7147-00, 1:100 dilution) anti-BLBP (rabbit, Abcam, ab32423, 1:500 dilution). anti-doublecortin (rabbit, Cell Signaling, 4604, 1:200 dilution). anti-doublecortin (guinea Pig, Millipore, ab2253, 1:200 dilution). anti-DLX2 (mouse, 3B6 clone, Santa Cruz, sc-393879, 1:200 dilution). anti-EMX1 (mouse, 3H2 clone, Santa Cruz, sc-398115, 1:200 dilution). anti-FOXP2 (rabbit, Millipore, ABE74, 1:500 dilution). anti-GFAP (chicken, Abcam, ab4674, 1;750 dilution). anti-GFP (chicken IgY, Aves labs, GFP-1020, 1:1000 dilution). anti-GSH2 (rabbit, Abcam, ab26255, 1:200 dilution). anti-IBA1 (goat, Abcam, ab5076, 1:250 dilution). anti-LHX6 (mouse, 6F3 clone, Santa Cruz, sc-271433, 1:200 dilution). anti-MEIS2 (mouse, 2F4 clone, Sigma,WH0004212M1, 1:500 dilution). anti-Mki67 (rabbit, Abcam, ab15580, 1:500 dilution). anti-NeuN (chicken, Millipore, ABN91, 1:500 dilution). anti-NKX2.1 (rabbit, Santa Cruz, sc-13040, 1:100 dilution). anti-Olig2 (rabbit, Millipore, AB9610, 1:200 dilution). anti-MBP (rat, 2D1 clone, Millipore, MAB386, 1:200 dilution). anti-PAX6 (mouse, 1B5 clone, Abcam, ab78545, 1:200 dilution). anti-PROX1 (goat, R&D, AF2727, 1:200 dilution). anti-PSA-NCAM (mouse, 5A5 clone, Millipore, MAB5324, 1:500 dilution). anti-SCGN (mouse, EPR23393-30 clone, Abcam, ab244219, 1:500 dilution) anti-SP8 (goat, Santa Cruz, sc-10466, 1:100 dilution). anti-TSHZ1 (rabbit, Abcam, ab140196, 1:200 dilution). anti-CXCR4 (rabbit, Abcam, ab124824, 1:100 dilution). anti-VLDLR (rabbit, Abcam, ab203271, 1:200 dilution). anti-PV (rabbit, Swant, PV27, 1:500 dilution). anti-PV (mouse, 1B2 clone, Swant, PV235, 1:200 dilution). anti-SST (rat, Abcam, ab30788, 1:200 dilution). anti-VIP (rabbit, Abcam, ab22736, 1:200 dilution). anti-CALB2 (rabbit, Swant, 7697, 1:200 dilution). anti-CALB2 (guinea Pig, Swant, CRgp7, 1:200 dilution). .. Biotin-SP-AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 715065151, 1:250 dilution).

Flow Cytometry:

Magnetic Resonance Imaging:

Staining:

Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation
Article Snippet: Specifically, RNAscope probes Mm-Lef1 (catalog no. 441861) and Mm-Msx1 (catalog no. 421841, Advanced Cell Diagnostics) were applied. .. Before 4′,6-diamidino-2-phenylindole (DAPI) staining, the slides were blocked in blocking buffer (5% donkey serum/0.1% Triton X-100 in PBS) for 1 hour at room temperature and counterstained with anti–COUP-TFII (1:200, R&D Systems, catalog no. PP-H7147-00), anti-AMH (1:500, Santa Cruz Biotechnology, catalog no. sc-6886), or anti-FOXL2 (1:200, Novus Biological, catalog no. NB100-1277) antibodies diluted in blocking buffer overnight at 4°C. .. The next day, the slides were washed and incubated with donkey anti-mouse (1:200, Invitrogen, catalog no. A10037) and donkey anti-goat (1:200, Invitrogen, catalog no. A21447) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.

Article Title: Single-nucleus multiomics of murine gonadal cells reveals transcriptional regulatory network underlying supporting lineage differentiation
Article Snippet: Specifically, RNAscopeTM probes Mm-Lef1 (Cat# 441861) and Mm-Msx1 (Cat# 421841, Advanced Cell Diagnostics) were applied. .. Before DAPI staining, the slides were blocked in blocking buffer (5% donkey serum/0.1% Triton X-100 in PBS) for 1 h at room temperature and counterstained with anti-COUP-TFII (1:200, R&D Systems, Cat# PP-H7147-00), anti-AMH (1:500, Santa Cruz, Cat# sc-6886), or anti-FOXL2 (1:200, Novus Biological, Cat# NB100-1277) antibodies diluted in blocking buffer overnight at 4 °C. .. The next day, the slides were washed and incubated with Donkey anti-mouse (1:200, Invitrogen, Cat# A10037) and Donkey anti-goat (1:200, Invitrogen, Cat# A21447) secondary antibodies diluted in blocking buffer incubation for 1 h at room temperature.

Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation.
Article Snippet: Specifically, RNAscope probes Mm- Lef1 (catalog no. 441861) and Mm- Msx1 (catalog no. 421841, Advanced Cell Diagnostics) were applied. .. Before 4′,6- diamidino- 2- phenylindole (DAPI) staining, the slides were blocked in blocking buffer (5% donkey serum/0.1% Triton X- 100 in PBS) for 1 hour at room temperature and counterstained with anti–COUP- TFII (1:200, R&D Systems, catalog no. PP- H7147- 00), anti- AMH (1:500, Santa Cruz Biotechnology, catalog no. sc- 6886), or anti- FOXL2 (1:200, Novus Biological, catalog no. NB100- 1277) antibodies diluted in blocking buffer overnight at 4°C. .. The next day, the slides were washed and incubated with donkey anti- mouse (1:200, Invitrogen, catalog no. A10037) and donkey antigoat (1:200, Invitrogen, catalog no. A21447) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.

Blocking Assay:

Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation
Article Snippet: .. Tissues were incubated overnight at 4°C in blocking buffer containing anti–COUP-TFII (1:200, R&D Systems, catalog no. PP-H7147-00), anti-FOXL2 (1:200, Novus Biological, catalog no. NB100-1277), and anti-LEF1 (1:200, catalog no. PA5-119910) antibodies. .. The next day, tissues were washed three times for 1 hour each in blocking buffer at 4°C, followed by incubation with donkey anti-mouse (1:200, Invitrogen, catalog no. A10037), donkey anti-goat (1:200, Invitrogen, catalog no. A21447), and donkey anti-rabbit (1:200, Invitrogen, catalog no. A21206) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.

Incubation:

Article Title: Single-nucleus multiomics of murine gonads reveals transcriptional regulatory network underlying supporting lineage differentiation
Article Snippet: .. Tissues were incubated overnight at 4°C in blocking buffer containing anti–COUP-TFII (1:200, R&D Systems, catalog no. PP-H7147-00), anti-FOXL2 (1:200, Novus Biological, catalog no. NB100-1277), and anti-LEF1 (1:200, catalog no. PA5-119910) antibodies. .. The next day, tissues were washed three times for 1 hour each in blocking buffer at 4°C, followed by incubation with donkey anti-mouse (1:200, Invitrogen, catalog no. A10037), donkey anti-goat (1:200, Invitrogen, catalog no. A21447), and donkey anti-rabbit (1:200, Invitrogen, catalog no. A21206) secondary antibodies diluted in blocking buffer incubation for 1 hour at room temperature.

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